发明申请
US20110151444A1 METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR
审中-公开
用于检测RNA分子的方法，与其相关的试剂盒及其用途
- 专利标题： METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR
- 专利标题（中）： 用于检测RNA分子的方法，与其相关的试剂盒及其用途
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申请号： US12968322申请日： 2010-12-15
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公开(公告)号： US20110151444A1公开(公告)日： 2011-06-23
- 发明人: Andreas Albers , Waltraud Ankenbauer , Frank Bergmann , Stefanie Froehner , Dieter Heindl
- 申请人： Andreas Albers , Waltraud Ankenbauer , Frank Bergmann , Stefanie Froehner , Dieter Heindl
- 优先权： EP09015714.0 20091218
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
Described is a method for the detection of a RNA molecule, the method involving the steps of providing a sample containing the RNA molecule; hybridizing to the RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. Also described is a kit containing a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety.
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