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1.发明申请METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR 审中-公开用于检测RNA分子的方法，与其相关的试剂盒及其用途公开(公告)号：US20110151444A1
公开(公告)日：2011-06-23
申请号：US12968322
申请日：2010-12-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/207 , C12Q2525/186 , C12Q2525/155
摘要： Described is a method for the detection of a RNA molecule, the method involving the steps of providing a sample containing the RNA molecule; hybridizing to the RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. Also described is a kit containing a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety.
摘要翻译： 描述了用于检测RNA分子的方法，该方法包括提供含有RNA分子的样品的步骤; 与RNA分子杂交第一多核苷酸; 扩展第一多核苷酸以产生第一链cDNA; 将第二多核苷酸与第一链cDNA杂交; 延伸第一链cDNA以产生延伸反应产物; 通过聚合酶链反应扩增延伸反应产物; 并通过实时荧光读数检测扩增产物。 还描述了包含本发明中定义的第一和第二多核苷酸的试剂盒，一组dNTP，逆转录酶和检测部分。
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公开(公告)号：US20090269766A1
公开(公告)日：2009-10-29
申请号：US12406450
申请日：2009-03-18
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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公开(公告)号：US20100258742A1
公开(公告)日：2010-10-14
申请号：US12748535
申请日：2010-03-29
CPC分类号： C12Q1/6818 , C12Q1/6848 , C12Q2565/101 , C12Q2563/131 , C12Q2545/101 , C12Q2537/157 , C12Q2565/501
摘要： The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye.
摘要翻译： 本发明提供了基于设计成能够彼此能量传递的染料的组成比控制的试剂盒和方法。 更详细地，利用本发明的方法，可以验证包含第一染料的第一组分与包含第二染料的第二组分的混合比。
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公开(公告)号：US20090181401A1
公开(公告)日：2009-07-16
申请号：US12407339
申请日：2009-03-19
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应， 温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用 所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合 或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光 至少在多个放大循环之后。
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公开(公告)号：US20050037410A1
公开(公告)日：2005-02-17
申请号：US10903992
申请日：2004-07-30
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12Q1/6818 , C12Q2561/113 , C12Q2531/113 , C12Q2521/319
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles.
摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法和组合物，其包括在热稳定性DNA聚合酶，耐热双链依赖性3'-5'的存在下使所述靶核酸进行实时PCR扩增反应， 温度最高在37℃以上的外切核酸酶，一对扩增引物，脱氧核苷三磷酸，携带第一标记和第二标记的检测寡核苷酸，所述第一标记当用 所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记与所述检测寡核苷酸的3'末端结合，其特征还在于另一个标记在内部结合 或在所述检测寡核苷酸的5'末端，并监测所述荧光报告的荧光 至少在多个放大循环之后。
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公开(公告)号：US08674080B2
公开(公告)日：2014-03-18
申请号：US12748535
申请日：2010-03-29
CPC分类号： C12Q1/6818 , C12Q1/6848 , C12Q2565/101 , C12Q2563/131 , C12Q2545/101 , C12Q2537/157 , C12Q2565/501
摘要： The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye.
摘要翻译： 本发明提供了基于设计成能够彼此能量传递的染料的组成比控制的试剂盒和方法。 更详细地，利用本发明的方法，可以验证包含第一染料的第一组分与包含第二染料的第二组分的混合比。
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公开(公告)号：US20130109060A1
公开(公告)日：2013-05-02
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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公开(公告)号：US07910720B2
公开(公告)日：2011-03-22
申请号：US12547013
申请日：2009-08-25
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Eva Walter , Renate Kolb
CPC分类号： C07H21/00 , C12Q1/6848 , C12Q2527/125
摘要： The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
摘要翻译： 本发明涉及一种包含结构[Xx-（CH2）m-磷酸-Yy] n结构的新化合物，其特征在于，3和nlE; m＆nlE; 6,30＆lt; n; n＆nlE; 60，每个x和y各自独立地表示 其他0或1，每个X和Y彼此独立地具有任何光度测量实体; 条件是末端X也可以是-OH基或磷酸基，并且进一步规定，末端Y也可以是-OH基。 这种化合物可以用作基于PCR扩增核酸的合适的热启动添加剂。
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公开(公告)号：US07122355B2
公开(公告)日：2006-10-17
申请号：US10192902
申请日：2002-07-11
申请人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
发明人： Waltraud Ankenbauer , Dieter Heindl , Frank Laue , Andreas Huber
IPC分类号： C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/319
摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
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公开(公告)号：US08592184B2
公开(公告)日：2013-11-26
申请号：US13711846
申请日：2012-12-12
申请人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
发明人： Dieter Heindl , Waltraud Ankenbauer , Frank Laue
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。
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