发明申请
- 专利标题: CELLULASE VARIANTS
- 专利标题(中): 纤维素酶变体
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申请号: US13162636申请日: 2011-06-17
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公开(公告)号: US20110250674A1公开(公告)日: 2011-10-13
- 发明人: Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
- 申请人: Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
- 申请人地址: DK Bagsvaerd
- 专利权人: Novozymes A/S
- 当前专利权人: Novozymes A/S
- 当前专利权人地址: DK Bagsvaerd
- 优先权: DK1013/96 19960917
- 主分类号: C12N9/42
- IPC分类号: C12N9/42
摘要:
The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
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