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公开(公告)号：US08017372B2
公开(公告)日：2011-09-13
申请号：US11830063
申请日：2007-07-30
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christiansen , Bo Damgaard , Claus Von der Osten
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号：US20090170747A1
公开(公告)日：2009-07-02
申请号：US12394202
申请日：2009-02-27
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号：US20080206836A1
公开(公告)日：2008-08-28
申请号：US11830063
申请日：2007-07-30
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号：US07993898B2
公开(公告)日：2011-08-09
申请号：US12394202
申请日：2009-02-27
申请人： Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christensen , Bo Damgaard , Claus Von der Osten
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
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公开(公告)号：US5997584A
公开(公告)日：1999-12-07
申请号：US116015
申请日：1998-07-15
申请人： Bente Konggaard Andersen , Kim Borch , Masanobu Abo , Bo Damgaard
发明人： Bente Konggaard Andersen , Kim Borch , Masanobu Abo , Bo Damgaard
CPC分类号： C11D3/38645 , C11D3/38636 , D06M16/003 , D06P5/02 , D06P5/137 , D06P5/158 , D06M2200/35
摘要： This invention relates to a method of reducing the pilling propensity or colour clarity of polyester fabrics and/or garments, which method comprises treating the fabric with a polyester hydrolytic enzyme and a detergent.
摘要翻译： 本发明涉及一种降低聚酯织物和/或衣物的起球倾向或着色透明度的方法，该方法包括用聚酯水解酶和洗涤剂处理织物。
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公开(公告)号：US20110250674A1
公开(公告)日：2011-10-13
申请号：US13162636
申请日：2011-06-17
申请人： Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Vilbour Andersen , Martin Schülein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N9/42
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号：US20050009166A1
公开(公告)日：2005-01-13
申请号：US10919195
申请日：2004-08-16
申请人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人： Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/21 , C12N9/42 , C12R1/645 , C12S11/00 , C12S99/00 , D06L4/40 , D06M16/00 , D21B1/02 , D21C5/02 , D21H21/24 , C07H21/04 , C12N15/74
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号：US20120289450A1
公开(公告)日：2012-11-15
申请号：US13471757
申请日：2012-05-15
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).
摘要翻译： 本发明涉及通过取代，插入和/或缺失衍生自亲代纤维素酶（即内切-β-1,4-葡聚糖酶）的纤维素酶变体，即内切-β-1,4-葡聚糖酶变体，该变体 具有催化核心结构域，其中第5位的变体含有丙氨酸残基（A），丝氨酸残基（S）或苏氨酸残基（T）; 在8位保持苯丙氨酸残基（F）或酪氨酸残基（Y）; 位置9含有苯丙氨酸残基（F），色氨酸残基（W）或酪氨酸残基（Y）; 在10位保持天冬氨酸残基（D）; 并且在位置121处保持天冬氨酸残基（D）。
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