发明申请
- 专利标题： Bacterial Mediated Delivery of Nuclear Protein Into Pluripotent and Differentiated Cells
- 专利标题（中）： 细菌介导的核蛋白递送到多能细胞和分化细胞
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申请号： US12842448申请日： 2010-07-23
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公开(公告)号： US20120021517A1公开(公告)日： 2012-01-26
- 发明人: SHOUGUANG JIN , CANDACE BICHSEL
- 申请人： SHOUGUANG JIN , CANDACE BICHSEL
- 申请人地址： US FL GAINESVILLE
- 专利权人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
- 当前专利权人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
- 当前专利权人地址： US FL GAINESVILLE
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C12N1/21
摘要：
A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.
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