发明申请
US20120028257A1 Modified luciola cruciata luciferase gene and protein
有权
修饰的luciola cruciata萤光素酶基因和蛋白质
- 专利标题： Modified luciola cruciata luciferase gene and protein
- 专利标题（中）： 修饰的luciola cruciata萤光素酶基因和蛋白质
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申请号： US12928337申请日： 2010-12-09
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公开(公告)号： US20120028257A1公开(公告)日： 2012-02-02
- 发明人: Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
- 申请人： Ying Jiang , Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
- 专利权人： Marker Gene Technologies, Inc.
- 当前专利权人： Marker Gene Technologies, Inc.
- 主分类号： C12Q1/66
- IPC分类号： C12Q1/66 ; C12N15/12 ; C12Q1/68
摘要：
A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
公开/授权文献
- US08450054B2 Modified Luciola cruciata luciferase gene and protein 公开/授权日：2013-05-28
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