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公开(公告)号：US20120028257A1
公开(公告)日：2012-02-02
申请号：US12928337
申请日：2010-12-09
CPC分类号： C12Y113/12007 , C12N9/0069 , C12Q1/008 , C12Q1/66
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。 描述了使用这种新酶测量各种生物代谢功能的测定法。
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公开(公告)号：US08450054B2
公开(公告)日：2013-05-28
申请号：US12928337
申请日：2010-12-09
CPC分类号： C12Y113/12007 , C12N9/0069 , C12Q1/008 , C12Q1/66
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。 描述了使用这种新酶测量各种生物代谢功能的测定法。
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公开(公告)号：US20120034634A1
公开(公告)日：2012-02-09
申请号：US12800830
申请日：2010-05-24
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。
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公开(公告)号：US08206961B2
公开(公告)日：2012-06-26
申请号：US12800830
申请日：2010-05-24
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。
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公开(公告)号：US07723502B2
公开(公告)日：2010-05-25
申请号：US12287561
申请日：2008-10-10
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。
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6.发明申请公开(公告)号：US20100093029A1
公开(公告)日：2010-04-15
申请号：US12287561
申请日：2008-10-10
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译： 基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性 ，被披露。 与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。
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