发明申请
- 专利标题： CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS
- 专利标题（中）： 用于上皮干细胞的培养基和含有干细胞的组织
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申请号： US13147163申请日： 2010-02-03
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公开(公告)号： US20120028355A1公开(公告)日： 2012-02-02
- 发明人: Toshiro Sato , Johannes Carolus Clevers , Meritxell Huch Ortega
- 申请人： Toshiro Sato , Johannes Carolus Clevers , Meritxell Huch Ortega
- 申请人地址： NL Amsterdam
- 专利权人： Koninklijke Nederlandse Akademie van Wetenschappen
- 当前专利权人： Koninklijke Nederlandse Akademie van Wetenschappen
- 当前专利权人地址： NL Amsterdam
- 优先权： EP09151970.2 20090203; EP09171831.2 20090930
- 国际申请： PCT/NL10/00017 WO 20100203
- 主分类号： C12N5/071
- IPC分类号： C12N5/071
摘要：
The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.
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