公开(公告)号：US20240360471A1

公开(公告)日：2024-10-31

申请号：US18457258

申请日：2023-08-28

申请人： EDITAS MEDICINE, INC.

发明人： Jennifer Leah GORI ,  Luis A. BARRERA

IPC分类号： C12N15/85 ,  C12N5/071 ,  C12N9/22 ,  C12N15/113

CPC分类号： C12N15/85 ,  C12N5/0602 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/322 ,  C12N2510/00 ,  C12N2800/80

摘要： Provided herein are CRISPR/Cas-related methods and components for editing a target nucleic acid sequence in a HBG1 and/or HBG2 gene regulatory region, and applications thereof in connection with methods of increasing expression of fetal hemoglobin and treating β-hemoglobinopathies including sickle cell disease and β-thalassemia.

公开(公告)号：US12129492B2

公开(公告)日：2024-10-29

申请号：US17343411

申请日：2021-06-09

申请人： D. Lansing Taylor ,  Albert Gough ,  Larry Vernetti

发明人： D. Lansing Taylor ,  Albert Gough ,  Larry Vernetti

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  G01N33/50

CPC分类号： C12N5/0697 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M35/08 ,  C12N5/067 ,  G01N33/5067 ,  G01N33/5082 ,  B01L2300/0636 ,  C12N2502/11 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2533/50 ,  C12N2533/54

摘要： Microfluidic devices for modeling three-dimensional tissue structures and methods for making and using the same are described herein.

公开(公告)号：US12128428B2

公开(公告)日：2024-10-29

申请号：US17296776

申请日：2019-12-09

申请人： ALFA LAVAL CORPORATE AB

发明人： Kasper Höglund ,  Peter Thorwid

IPC分类号： B04B9/12 ,  B04B1/08 ,  B04B7/14 ,  B04B11/02 ,  C12M1/00 ,  C12N5/071

CPC分类号： B04B9/12 ,  B04B1/08 ,  B04B7/14 ,  B04B11/02 ,  C12M47/02 ,  C12N5/0682

摘要： A centrifugal separator for separation of a cell culture mixture includes a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing opposite and arranged so that a separated cell phase is discharged at the rotational axis. The rotatable assembly includes an exchangeable separation insert and a rotatable member.

公开(公告)号：US20240352419A1

公开(公告)日：2024-10-24

申请号：US18025588

申请日：2022-12-02

申请人： NUWACELL BIOTECHNOLOGIES CO., LTD.

发明人： Junying YU ,  Tao ZHOU ,  Changqing TIAN ,  Yali GAO ,  Ying ZHANG

IPC分类号： C12N5/0783 ,  C12N5/071 ,  C12N5/0789

CPC分类号： C12N5/0646 ,  C12N5/0647 ,  C12N5/069 ,  C12N2500/30 ,  C12N2500/90 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2506/45 ,  C12N2513/00

摘要： The present disclosure provides, inter alia, methods and compositions for differentiation of pluripotent stem cells and derived hematopoietic lineage cells including hemogenic endothelial cells, hematopoietic progenitor cells and natural killer cells. The differentiation efficiency for the hemogenic endothelial cells, the hematopoietic progenitor cells and the natural killer cells can be improved by using the methods and compositions of this disclosure described herein.

公开(公告)号：US20240352406A1

公开(公告)日：2024-10-24

申请号：US18639518

申请日：2024-04-18

申请人： Cellum Biomedical, Inc.

发明人： Richard C. Jin

IPC分类号： C12N5/00 ,  A61K9/06 ,  A61K9/16 ,  A61K47/36 ,  C12N5/071 ,  C12N5/077

CPC分类号： C12N5/0018 ,  A61K9/06 ,  A61K9/1658 ,  A61K47/36 ,  C12N5/0625 ,  C12N5/0654 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/148 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/19 ,  C12N2501/22 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/2308 ,  C12N2501/25 ,  C12N2502/1142 ,  C12N2502/1323

摘要： The present invention is drawn, in part, to biocompatible compositions comprising a biocompatible polymer matrix and conditioned cell medium comprising i) a cell culture medium and ii) one or more agents synthesized by and secreted from one or more cells cultured in the cell culture medium, as well as therapeutic uses thereof, particularly in modulating bone and/or gum tissue growth.

公开(公告)号：US12123024B1

公开(公告)日：2024-10-22

申请号：US18410576

申请日：2024-01-11

申请人： Andrea D. Raymond ,  Madhavan P. Nair ,  Arti Vashist ,  Mickensone Andre

发明人： Andrea D. Raymond ,  Madhavan P. Nair ,  Arti Vashist ,  Mickensone Andre

IPC分类号： C12N5/071 ,  C12N5/00

CPC分类号： C12N5/0686 ,  C12N5/0081 ,  C12N2500/50 ,  C12N2501/998

摘要： The subject invention provides compositions and methods for isolating exosomes from, for example, biological fluid samples, cell cultures and/or cultured medium. Provided are Absolute Precipitation of Exosomes (APEX) reagents and precipitation techniques to isolate exosomes from various EV subsets, cells, dead cells, and/or cell debris. Advantageously, APEX is a rapid, effortless, inexpensive, high-recovery method to extract exosomes. This method is also faster and purer at isolating exosomes from other precipitation techniques.

公开(公告)号：US20240344958A1

公开(公告)日：2024-10-17

申请号：US18628507

申请日：2024-04-05

申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Utkan Demirci ,  Waseem Asghar

IPC分类号： G01N15/10 ,  B01L3/00 ,  C12M3/00 ,  C12N5/071 ,  C12N5/076 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01

CPC分类号： G01N15/10 ,  B01L3/502753 ,  C12N5/061 ,  C12N5/0612 ,  G01N1/4005 ,  G01N15/00 ,  C12M21/06 ,  G01N2001/4088 ,  G01N2015/0003 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N2800/367

摘要： A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

公开(公告)号：US20240344033A1

公开(公告)日：2024-10-17

申请号：US18682573

申请日：2021-08-18

申请人： TOHOKU UNIVERSITY

发明人： Takahiro ARIMA ,  Shun SHIBATA ,  Hiroaki OKAE

IPC分类号： C12N5/071 ,  G09B23/30

CPC分类号： C12N5/0682 ,  G09B23/306 ,  C12N2502/02 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2533/54

摘要： There is provided an endometrial model including: a stromal extracellular matrix-containing gel; and endometrial epithelial cells, wherein at least some of the endometrial epithelial cells are exposed on a surface of the gel, and wherein the at least some of the endometrial epithelial cells exposed on the surface of the gel continuously form a luminal structure and a non-luminal structure. Also, a method for preparing the endometrial model is also provided. Further, an endometrial implantation model including: the endometrial model and an embryonic cell is provided.

公开(公告)号：US12115195B2

公开(公告)日：2024-10-15

申请号：US17363898

申请日：2021-06-30

申请人： INCUBE LABS, LLC

发明人： Mir Imran

IPC分类号： A61K35/38 ,  A61K35/28 ,  C12N5/071

CPC分类号： A61K35/38 ,  A61K35/28 ,  C12N5/0679 ,  C12N2502/025 ,  C12N2502/03 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/23 ,  C12N2506/1346 ,  C12N2506/45 ,  C12N5/0679 ,  C12N2502/1352 ,  C12N5/0679 ,  C12N2502/03 ,  C12N5/0679 ,  C12N2506/1346 ,  C12N5/0679 ,  C12N2506/03

摘要： Various embodiments of the invention provide methods of treating diabetes and other glucose regulation disorders. In one embodiment, the method comprises removing L-cells from a donor, obtaining stem cells from a patient, and culturing the L-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived L-cells (SCDLC). An amount of the SCDLC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food. In another embodiment, the method comprises removing K-cells from a donor, obtaining stem cells from a patient, and culturing the K-cells in the presence of the stem cells under conditions such that the stem cells differentiate into stem cell-derived K-cells (SCDKC). An amount of the SCDKC is introduced into the patient sufficient to cause a lowering of the patient's blood glucose level after ingestion of food.

公开(公告)号：US12111308B2

公开(公告)日：2024-10-08

申请号：US17147022

申请日：2021-01-12

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA

发明人： Lalitha Madhavan

IPC分类号： G01N33/50 ,  C12N5/071

CPC分类号： G01N33/5091 ,  C12N5/0625 ,  G01N33/5044 ,  G01N2800/2835 ,  G01N2800/52 ,  G01N2800/60 ,  G01N2800/7009

摘要： Primary skin fibroblasts obtained from individuals diagnosed with late-onset sporadic Parkinson's disease (PD), were compared to healthy age-matched controls. Fibroblasts from PD subjects had higher growth rates, and appeared distinctly different in terms of morphology and spatial organization in culture, compared to control cells. The PD fibroblasts also exhibited significantly compromised mitochondrial structure and function when assessed via morphological and oxidative phosphorylation assays. Additionally, an increase in baseline macroautophagy levels was seen in cells from PD subjects. Exposure of the skin fibroblasts to physiologically relevant stress, specifically ultraviolet irradiation (UVA), further exaggerated the autophagic dysfunction in the PD cells. Moreover, the PD fibroblasts accumulated higher levels of reactive oxygen species (ROS) coupled with lower cell viability upon UVA treatment. These results highlight primary skin fibroblasts as a patient-relevant model that captures fundamental PD molecular mechanisms, and enable their utility as diagnostic and prognostic biomarkers for PD.