The present invention discloses a method of forming an alignment film for use in a liquid crystal display (LCD). The method includes: Provide a glass substrate and dispose an ITO film on the glass substrate. Next, set the thickness of the alignment film. Next, determine the spray volume of thin film material droplets based on the length and the width of the glass substrate. Next, spray a plurality of thin film material droplets in rows on the glass substrate through a plurality of nozzles of an inkjet head. The thin film material droplets are distributed in an approximate equilateral triangular pattern. The thin film material droplets in the shape of an approximate equilateral triangle can reduce instability when spreading on the glass substrate, achieving a target of optimized coatings. Finally, process an alignment material thin film which is formed after the thin film material droplets spread to form an alignment film.