发明申请
- 专利标题: ULTRA SENSITIVE METHOD FOR IN SITU DETECTION OF NUCLEIC ACIDS
- 专利标题(中): 用于检测核酸的超敏感方法
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申请号: US13278353申请日: 2011-10-21
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公开(公告)号: US20120100540A1公开(公告)日: 2012-04-26
- 发明人: XINGYONG WU , Huei-Yu Fay WANG , Nan SU , Li-Chong WANG , Yuling LUO
- 申请人: XINGYONG WU , Huei-Yu Fay WANG , Nan SU , Li-Chong WANG , Yuling LUO
- 申请人地址: US CA Hayward
- 专利权人: Advanced Cell Diagnostics, Inc.
- 当前专利权人: Advanced Cell Diagnostics, Inc.
- 当前专利权人地址: US CA Hayward
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.