公开(公告)号：US20120100540A1

公开(公告)日：2012-04-26

申请号：US13278353

申请日：2011-10-21

申请人： XINGYONG WU ,  Huei-Yu Fay WANG ,  Nan SU ,  Li-Chong WANG ,  Yuling LUO

发明人： XINGYONG WU ,  Huei-Yu Fay WANG ,  Nan SU ,  Li-Chong WANG ,  Yuling LUO

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

摘要： Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.

摘要翻译： 本发明公开了一种基于RNA方法和一般ISH信号扩增方法的组合来原位检测一种或多种靶核酸的方法。 这种新方法产生高信号强度，同时保持低信号放大的背景噪声。 结果可以一贯重现，该方法可以方便地用于常规诊所诊断。 此外，本发明涉及一种试剂盒，其包含用于灵敏检测一种或多种靶核酸的RNA检测和一般ISH信号扩增测定的组分。

公开(公告)号：US20140178869A1

公开(公告)日：2014-06-26

申请号：US13843408

申请日：2013-03-15

申请人： Xiao-Jun MA ,  Yuling LUO ,  Raymond Tubbs

发明人： Xiao-Jun MA ,  Yuling LUO ,  Raymond Tubbs

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C07K16/00 ,  C07K2317/515 ,  C12Q1/6886 ,  C12Q2600/158

摘要： The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by obtaining a sample of B cells from a subject; conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells.

摘要翻译： 本发明提供一种通过从受试者获得B细胞样品来检测B细胞中免疫球蛋白轻链限制和克隆性的方法; 使用（i）设计为与免疫球蛋白κ恒定区（IGKCR）RNA特异性杂交的至少一个探针组进行样品上的双链原位杂交测定; 和（ii）设计成与免疫球蛋白λ链恒定区（IGLCR）RNA特异性杂交的至少一个探针组; 检测与杂交的IGKCR探针相关的信号和与样品中B细胞群中的杂交IGLCR探针相关的信号; 以及确定在B细胞群体中的各个细胞内与杂交的IGKCR探针和杂交的IGLCR探针相关联的信号模式，其中各个细胞内的信号模式指示存在或不存在B细胞的轻链限制和克隆。