发明申请
- 专利标题： EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF
- 专利标题（中）： 用动物细胞生产大量外源基因衍生蛋白的表达载体及其用途
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申请号： US13140905申请日： 2009-12-22
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公开(公告)号： US20120122083A1公开(公告)日： 2012-05-17
- 发明人: Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
- 申请人： Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
- 优先权： JP2008-325690 20081222
- 国际申请： PCT/JP2009/071326 WO 20091222
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12Q1/68 ; C12N15/85
摘要：
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
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