A microfluidic device to meter an accurate amount of fluid is provided. The microfluidic device includes a chamber configured to accommodate a fluid therein, an inlet channel connected to the chamber to supply the fluid into the chamber, and an outlet channel connected to the chamber and provided separately from the inlet channel. The outlet channel is configured such that after the chamber is filled with a predetermined amount of the fluid introduced through the inlet channel, any additional amount of the fluid introduced into the chamber in excess of the predetermined amount exits the chamber through the outlet channel.