发明申请
- 专利标题: METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT
- 专利标题(中): 普通小麦定性和定量检测方法
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申请号: US13518298申请日: 2010-12-17
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公开(公告)号: US20120264128A1公开(公告)日: 2012-10-18
- 发明人: Kazumi Kitta , Satoshi Furui , Junichi Mano , Yasuyuki Matsuoka , Shinichiro Arami , Megumi Sato , Hiroyuki Haraguchi , Youichi Kurimoto , Shinjiro Imai , Keiko Tanaka
- 申请人: Kazumi Kitta , Satoshi Furui , Junichi Mano , Yasuyuki Matsuoka , Shinichiro Arami , Megumi Sato , Hiroyuki Haraguchi , Youichi Kurimoto , Shinjiro Imai , Keiko Tanaka
- 申请人地址: JP Chiyoda-ku, Tokyo JP Shibuya-ku, Tokyo
- 专利权人: NISSHIN SEIFUN GROUP INC.,NIPPON FLOUR MILLS CO., LTD
- 当前专利权人: NISSHIN SEIFUN GROUP INC.,NIPPON FLOUR MILLS CO., LTD
- 当前专利权人地址: JP Chiyoda-ku, Tokyo JP Shibuya-ku, Tokyo
- 优先权: JP2009-289340 20091221
- 国际申请: PCT/JP2010/072806 WO 20101217
- 主分类号: G01N21/64
- IPC分类号: G01N21/64 ; C07H21/04 ; C12Q1/68
摘要:
Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.
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