公开(公告)号：US09273362B2

公开(公告)日：2016-03-01

申请号：US13517453

申请日：2010-12-17

申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Shinjiro Imai ,  Keiko Tanaka ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto

发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Shinjiro Imai ,  Keiko Tanaka ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2545/113

摘要： Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule.

公开(公告)号：US20120264128A1

公开(公告)日：2012-10-18

申请号：US13518298

申请日：2010-12-17

申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

IPC分类号： G01N21/64 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/6895

摘要： Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.

摘要翻译： 本发明公开了一种以高灵敏度，定性和/或定量方式特异性检测食品原料或加工食品中所含小麦品种中普通小麦的方法; 用于区分普通小麦和食品原料或加工食品中包含的普通小麦（例如硬粒小麦）以外的小麦品种的方法，并以定性和/或定量方式检测普通小麦; 以及引物组，核酸探针和检测试剂盒，其可以用于采用PCR方法的方法中。 具体公开的是：检测感兴趣的样品中普通小麦的发生的方法，包括使用从样品提取的核酸作为模板进行PCR方法，并使用包含SEQ ID NO所示的核苷酸序列的引物 5和包含SEQ ID NO：6所示核苷酸序列的引物，并检测PCR扩增产物的出现; 以及用于检测感兴趣样品中普通小麦的发生的方法，其包括使用从样品提取的核酸作为模板进行定量PCR方法，并使用包含SEQ ID NO：5所示核苷酸序列的引物 ，包含由SEQ ID NO：6表示的核苷酸序列的引物和包含SEQ ID NO：11所示的核苷酸序列的核酸探针，并定性和/或定量地检测普通小麦的发生。

公开(公告)号：US08865433B2

公开(公告)日：2014-10-21

申请号：US13518298

申请日：2010-12-17

申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto ,  Shinjiro Imai ,  Keiko Tanaka

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6895

摘要： Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.

摘要翻译： 本发明公开了一种以高灵敏度，定性和/或定量方式特异性检测食品原料或加工食品中所含小麦品种中普通小麦的方法; 用于区分普通小麦和食品原料或加工食品中包含的普通小麦（例如硬粒小麦）以外的小麦品种的方法，并以定性和/或定量方式检测普通小麦; 以及引物组，核酸探针和检测试剂盒，其可以用于采用PCR方法的方法中。 具体公开的是：检测感兴趣的样品中普通小麦的发生的方法，包括使用从样品提取的核酸作为模板进行PCR方法，并使用包含SEQ ID NO所示的核苷酸序列的引物 5和包含SEQ ID NO：6所示核苷酸序列的引物，并检测PCR扩增产物的出现; 以及用于检测感兴趣样品中普通小麦的发生的方法，其包括使用从样品提取的核酸作为模板进行定量PCR方法，并使用包含SEQ ID NO：5所示核苷酸序列的引物 ，包含由SEQ ID NO：6表示的核苷酸序列的引物和包含SEQ ID NO：11所示的核苷酸序列的核酸探针，并定性和/或定量地检测普通小麦的发生。

公开(公告)号：US20120258464A1

公开(公告)日：2012-10-11

申请号：US13517453

申请日：2010-12-17

申请人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Shinjiro Imai ,  Keiko Tanaka ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto

发明人： Kazumi Kitta ,  Satoshi Furui ,  Junichi Mano ,  Shinjiro Imai ,  Keiko Tanaka ,  Yasuyuki Matsuoka ,  Shinichiro Arami ,  Megumi Sato ,  Hiroyuki Haraguchi ,  Youichi Kurimoto

IPC分类号： C12Q1/68 ,  G06F19/20 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2545/113

摘要： Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule.

摘要翻译： 提供了通过聚合酶链式反应检测或定量测试样品中的小麦种类特异性DNA的方法。 该方法包括使用测试样品中的核酸分子或从测试样品提取的核酸分子作为模板来扩增具有鉴定为SEQ ID NO：1的核苷酸序列的部分序列的核酸分子的步骤，并使用 能够扩增部分序列的引物对和检测或定量扩增的核酸分子的步骤。