发明申请
US20120315620A1 Method and Apparatus for Discriminating Undifferentiated Pluripotent Stem Cells, and Automated Culture Method and System
有权
用于鉴别未分化多能干细胞的方法和装置，以及自动培养方法和系统
- 专利标题： Method and Apparatus for Discriminating Undifferentiated Pluripotent Stem Cells, and Automated Culture Method and System
- 专利标题（中）： 用于鉴别未分化多能干细胞的方法和装置，以及自动培养方法和系统
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申请号： US13500626申请日： 2010-10-07
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公开(公告)号： US20120315620A1公开(公告)日： 2012-12-13
- 发明人: Keizo Watakabe , Yohichi Nakamura , Osamu Ohji , Katsumi Nakashima
- 申请人： Keizo Watakabe , Yohichi Nakamura , Osamu Ohji , Katsumi Nakashima
- 申请人地址： JP Kobe-shi, HYOGO
- 专利权人： Kawasaki Jukogyo Kabushiki Kaisha
- 当前专利权人： Kawasaki Jukogyo Kabushiki Kaisha
- 当前专利权人地址： JP Kobe-shi, HYOGO
- 优先权： JP2009-235306 20091009
- 国际申请： PCT/JP2010/006007 WO 20101007
- 主分类号： G06K9/00
- IPC分类号： G06K9/00 ; C12M1/36 ; C12Q3/00
摘要：
Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.