公开(公告)号：US09008406B2

公开(公告)日：2015-04-14

申请号：US13500626

申请日：2010-10-07

申请人： Keizo Watakabe ,  Yohichi Nakamura ,  Osamu Ohji ,  Katsumi Nakashima

发明人： Keizo Watakabe ,  Yohichi Nakamura ,  Osamu Ohji ,  Katsumi Nakashima

IPC分类号： G06K9/00 ,  G01N33/50

CPC分类号： G01N33/5073 ,  G06K9/0014 ,  G06K9/00147

摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.

摘要翻译： 提供了能够区分分化多能干细胞的集落和未分化多能干细胞的集落的方法，以及用于自动培养未分化的多能干细胞的方法及其系统。 由分化多能干细胞组成的分化集落，由未分化多能干细胞组成的未分化菌落和多层堆叠多层干细胞的多层菌落通过在培养容器中加工包含多能干细胞的菌落的照片来鉴别。 具体而言，将亮度高于第一阈值A的菌落确定为差异菌落，将具有等于或低于第一阈值A且等于或高于第二阈值B的亮度的菌落确定为未分化菌落 ，并且将具有低于第二阈值B的亮度的菌落确定为多层菌落。

公开(公告)号：US20120315620A1

公开(公告)日：2012-12-13

申请号：US13500626

申请日：2010-10-07

申请人： Keizo Watakabe ,  Yohichi Nakamura ,  Osamu Ohji ,  Katsumi Nakashima

发明人： Keizo Watakabe ,  Yohichi Nakamura ,  Osamu Ohji ,  Katsumi Nakashima

IPC分类号： G06K9/00 ,  C12M1/36 ,  C12Q3/00

CPC分类号： G01N33/5073 ,  G06K9/0014 ,  G06K9/00147

摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.

摘要翻译： 提供了能够区分分化多能干细胞的集落和未分化多能干细胞的集落的方法，以及用于自动培养未分化的多能干细胞的方法及其系统。 由分化多能干细胞组成的分化集落，由未分化多能干细胞组成的未分化菌落和多层堆叠多层干细胞的多层菌落通过在培养容器中加工包含多能干细胞的菌落的照片来鉴别。 具体而言，将亮度高于第一阈值A的菌落确定为差异菌落，将具有等于或低于第一阈值A且等于或高于第二阈值B的亮度的菌落确定为未分化菌落 ，并且将具有低于第二阈值B的亮度的菌落确定为多层菌落。

公开(公告)号：US20130130228A1

公开(公告)日：2013-05-23

申请号：US13805767

申请日：2011-06-22

申请人： Keizo Watakabe ,  Takashi Sakurai ,  Osamu Ohji ,  Katsumi Nakashima

发明人： Keizo Watakabe ,  Takashi Sakurai ,  Osamu Ohji ,  Katsumi Nakashima

IPC分类号： C12Q1/04 ,  C12M1/36 ,  C12Q3/00

CPC分类号： C12M41/46

摘要： A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1

摘要翻译： 一种方法和装置能够确定培养容器上的菌落在培养多能干细胞的情况下是分化还是未分化，并且方法和装置实现未分化多能干细胞的快速培养。 通过在多能干细胞培养容器内部的图像处理的拍摄图像中的菌落的圆形度C，从其他类型的菌落中鉴定到仅含有未分化的多能干细胞的未分化菌落。 更具体地，当菌落的圆形度的第一阈值为C1，第二预定阈值为C2（C1

公开(公告)号：US08977031B2

公开(公告)日：2015-03-10

申请号：US13805767

申请日：2011-06-22

申请人： Keizo Watakabe ,  Takashi Sakurai ,  Osamu Ohji ,  Katsumi Nakashima

发明人： Keizo Watakabe ,  Takashi Sakurai ,  Osamu Ohji ,  Katsumi Nakashima

IPC分类号： G06K9/00 ,  G06T7/60 ,  C12M1/34

CPC分类号： C12M41/46

摘要： A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1

摘要翻译： 一种方法和装置能够确定培养容器上的菌落在培养多能干细胞的情况下是分化还是未分化，并且方法和装置实现未分化多能干细胞的快速培养。 通过在多能干细胞培养容器内部的图像处理的拍摄图像中的菌落的圆形度C，从其他类型的菌落中鉴定到仅含有未分化的多能干细胞的未分化菌落。 更具体地说，当菌落的圆形度的第一阈值为C1，第二预定阈值为C2（C1

公开(公告)号：US08265371B2

公开(公告)日：2012-09-11

申请号：US12296528

申请日：2007-04-12

申请人： Osamu Ohji ,  Toshihisa Doi

发明人： Osamu Ohji ,  Toshihisa Doi

IPC分类号： G06K9/00

CPC分类号： G01N33/5005 ,  C12M33/00 ,  C12M41/46

摘要： A novel method for evaluating cell detachment which can be used in passage operation and the like in culture of adhesive cells; a method of detaching a cultured cell utilizing the same; an apparatus for evaluating cell detachment for carrying out the aforementioned method for evaluating cell detachment; and an automated cell culture apparatus in which the apparatus is used are provided.

摘要翻译： 一种用于评价细胞分离的新方法，其可用于粘附细胞培养中的通过操作等; 使用该培养细胞分离培养细胞的方法; 用于评价细胞分离以进行上述评价细胞分离的方法的装置; 提供使用该装置的自动细胞培养装置。