发明申请
- 专利标题: Purification Method
- 专利标题(中): 净化方法
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申请号: US13575556申请日: 2011-02-03
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公开(公告)号: US20130012684A1公开(公告)日: 2013-01-10
- 发明人: Jens Buchardt
- 申请人: Jens Buchardt
- 申请人地址: DK Bagsvaerd
- 专利权人: Novo Nordisk A/S
- 当前专利权人: Novo Nordisk A/S
- 当前专利权人地址: DK Bagsvaerd
- 优先权: EP10153713.2 20100216
- 国际申请: PCT/EP2011/051570 WO 20110203
- 主分类号: C07K1/22
- IPC分类号: C07K1/22 ; C07K14/62 ; C07K14/745 ; C07K14/605 ; C07K14/755 ; C07K14/61
摘要:
The present invention provides a method for purification of a protein that is conjugated to an albumin binding moiety from a mixture comprising (i) said protein in said conjugated form and (ii) said protein in a form that is not conjugated to said albumin-binding moiety, the method comprising: (a) providing a solid support comprising a substance capable of specifically binding to the albumin binding moiety; (b) contacting said solid support of (a) with said mixture comprising protein and conjugated protein under suitable conditions for binding of the albumin binding moiety to the substance as defined in (a); and (c) eluting components bound to the solid support.
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