发明申请
- 专利标题： METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO SKELETAL MUSCLE OR SKELETAL MUSCLE PROGENITOR CELLS
- 专利标题（中）： 用于诱导巨噬细胞干细胞分化为骨骼肌或骨骼肌原细胞细胞的方法
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申请号： US13642650申请日： 2011-04-21
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公开(公告)号： US20130040387A1公开(公告)日： 2013-02-14
- 发明人: Toshio Heike , Tatsutoshi Nakahata , Tomonari Awaya
- 申请人： Toshio Heike , Tatsutoshi Nakahata , Tomonari Awaya
- 申请人地址： JP Kyoto-shi
- 专利权人： Kyoto University
- 当前专利权人： Kyoto University
- 当前专利权人地址： JP Kyoto-shi
- 国际申请： PCT/JP11/60340 WO 20110421
- 主分类号： C12N5/0775
- IPC分类号： C12N5/0775 ; C12N5/10
摘要：
A method for inducing the differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells is provided. Specifically, a method for producing artificial skeletal muscle or skeletal muscle progenitor cells from human pluripotent stem cells is provided, comprising the following steps of: (1) culturing human pluripotent stem cells by suspension culture; (2) culturing a cell population after suspension culture by adhesion culture; (3) dissociating cells after adhesion culture; and (4) culturing the dissociated cells by adhesion culture. Artificial skeletal muscle or induced skeletal muscle progenitor cells prepared by the method are also provided.
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