发明申请
US20130130228A1 METHOD AND DEVICE FOR IDENTIFYING MULTIPOTENT STEM CELL COLONY, AND METHOD AND DEVICE FOR AUTOMATICALLY CULTURING MULTIPOTENT STEM CELLS
有权
用于鉴定多个干细胞群的方法和装置，以及用于自动培养多个干细胞的方法和装置
- 专利标题： METHOD AND DEVICE FOR IDENTIFYING MULTIPOTENT STEM CELL COLONY, AND METHOD AND DEVICE FOR AUTOMATICALLY CULTURING MULTIPOTENT STEM CELLS
- 专利标题（中）： 用于鉴定多个干细胞群的方法和装置，以及用于自动培养多个干细胞的方法和装置
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申请号： US13805767申请日： 2011-06-22
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公开(公告)号： US20130130228A1公开(公告)日： 2013-05-23
- 发明人: Keizo Watakabe , Takashi Sakurai , Osamu Ohji , Katsumi Nakashima
- 申请人： Keizo Watakabe , Takashi Sakurai , Osamu Ohji , Katsumi Nakashima
- 申请人地址： JP Kobe-shi, Hyogo
- 专利权人： KAWASAKI JUKOGYO KABUSHIKI KAISHA
- 当前专利权人： KAWASAKI JUKOGYO KABUSHIKI KAISHA
- 当前专利权人地址： JP Kobe-shi, Hyogo
- 优先权： JP2010-145468 20100625
- 国际申请： PCT/JP2011/003574 WO 20110622
- 主分类号： C12Q1/04
- IPC分类号： C12Q1/04 ; C12M1/36 ; C12Q3/00
摘要：
A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1
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