发明申请
US20130288908A1 POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME
有权
可在无细胞翻译系统中显示FAB的多核苷酸结构，以及使用其制造和筛选FAB的方法
- 专利标题： POLYNUCLEOTIDE CONSTRUCT CAPABLE OF DISPLAYING FAB IN A CELL-FREE TRANSLATION SYSTEM, AND METHOD FOR MANUFACTURING AND SCREENING FAB USING SAME
- 专利标题（中）： 可在无细胞翻译系统中显示FAB的多核苷酸结构，以及使用其制造和筛选FAB的方法
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申请号： US13990654申请日： 2011-11-30
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公开(公告)号： US20130288908A1公开(公告)日： 2013-10-31
- 发明人: Yasuhiro Fujino , Risako Fujita , Kouichi Wada , Kotomi Oda , Takuya Ueda , Yoshihiro Shimizu , Takashi Kanamori
- 申请人： Yasuhiro Fujino , Risako Fujita , Kouichi Wada , Kotomi Oda , Takuya Ueda , Yoshihiro Shimizu , Takashi Kanamori
- 申请人地址： US Tokyo JP Osaka-shi, Osaka
- 专利权人： THE UNIVERSITY OF TOKYO,MITSUBISHI TANABE PHARMA CORPORATION
- 当前专利权人： THE UNIVERSITY OF TOKYO,MITSUBISHI TANABE PHARMA CORPORATION
- 当前专利权人地址： US Tokyo JP Osaka-shi, Osaka
- 优先权： JP2010268763 20101201
- 国际申请： PCT/JP2011/077725 WO 20111130
- 主分类号： G01N33/68
- IPC分类号： G01N33/68
摘要：
The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself, and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.
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