摘要:
The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.
摘要:
The present invention provides a method for producing a polypeptide, which comprises condensing precursors comprising an amino acid and an adaptor in the presence of ribosomes, rRNAs, a larger ribosomal subunit or ribosomal proteins, and an aromatic tertiary amine.
摘要:
A polymerization apparatus, comprises a polymerization vessel, a reflux condenser installed outside the polymerization vessel and a pipe connected between the reflux condenser and a wall of the polymerization vessel with an end thereof opening into a gaseous phase region inside the polymerization vessel, for returning the liquid condensate to the polymerization vessel, wherein said end projects from an inner surface of the wall of the polymerization vessel into the gaseous phase region. In polymerizing a monomer having an ethylenically unsaturated double bond using the polymerization apparatus, the quantity of heat removed by the reflux condenser is increased stepwise or continuously with progress of the polymerization, such that not less than 20% of the total reaction heat generated during the whole course of the polymerization is removed by the reflux condenser. With 100 or more repeated polymerization runs, polymer scale deposition inside the vessel is minimal even below the pipe end.
摘要:
A vinyl chloride polymer is produced by subjecting vinyl chloride or a monomer mixture made up mainly of vinyl chloride to suspension polymerization in an aqueous medium using a polymerization apparatus equipped with a polymerization vessel. The polymerization apparatus used comprises a polymerization vessel (1) having a substantially cylindrical inner space, said inner space of said polymerization vessel being provided with a plurality of baffles (5) comprising vertically extending pipes, and serpentine pipelines (6) each of which is placed between two adjacent baffles and is extended in a serpentine fashion along the polymerization vessel inner wall from the lower part to the upper part of the polymerization vessel, and a refrigerant being passed through said baffles and said serpentine pipelines. High-cooling performance can be obtained, a high-speed reaction in a large-sized polymerization vessel becomes possible, and a stable polymerization reaction can be carried out. A high-quality and uniform polymer that has few fish eyes and has a high bulk specific gravity can be obtained.
摘要:
A process for regenerating steel scrap by melting and refining it, the improvement wherein carbon is added to molten scrap steel melt in an amount sufficient to adjust the carbon content of the melt to 0.6 to 3.0%, and the scrap steel is refined by blowing oxygen therein. This method can also be applied to the production of steel from reduced iron.
摘要:
An induction melting furnace for melting metals or alloys including an auxiliary induction heating coil in a material charging part. The material charging part is additionally heated to promote dissolving of the material in molten metal.
摘要:
The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.
摘要:
The present invention provides a pyrazole derivative of the general formula (1), which has an excellent efficacy as an active component for a herbicide, an intermediate for the production thereof, processes for the production thereof, and a herbicide containing the derivative as an active ingredient.
摘要:
A preformed yarn useful for forming composite articles is disclosed which includes a core of a multiplicity of fibers, fine powder provided in the interstices between the fibers, and a flexible sleeve formed of a thermoplastic resin and surrounding the core. The fine powder is formed of a material which neither melts nor reacts with the thermoplastic resin at a temperature up to 450.degree. C. and at a temperature up to the melting point of the thermoplastic resin. The incorporation of the fine powder between the fibers is effected by injecting fine powder-containing air from a jet nozzle and blowing the air against a bundle of the fibers.
摘要:
A continuous process for the production of carbonaceous pitch, including heat-treating an aromatic heavy oil in a first thermal cracking zone for obtaining a first cracked product, and introducing the first cracked product into a second thermal cracking zone where it is thermally cracked by direct contact with a gaseous heat transfer medium to form distillable cracked components and a mesophase-containing pitch. The liquid phase in the second thermal cracking zone, including the mesophase-containing pitch, is discharged therefrom and separated into a mesophase-rich pitch and a matrix pitch having a low concentration of mesophase. The mesophase-rich pitch is recovered while at least a portion of the matrix pitch is recycled to the second thermal cracking zone. The distillable cracked components are stripped from the liquid phase in the second thermal cracking zone with the heat transfer medium, and the resulting gas phase is withdrawn overhead therefrom and then separated into a light fraction and a heavy fraction. The heavy fraction is fed to the first thermal cracking zone.