A method for manufacturing a color film substrate including the following steps is provided. A substrate is provided. A first conductive black matrix extending along a first direction is formed on the substrate. A color film layer is formed on the substrate, and the normal projections of the color film layer and of the first conductive black matrix on the substrate are not overlapping. A plurality of insulating spacers is formed on the first conductive black matrix, and the height of the insulating spacers is greater than the thickness of the color film layer. A second conductive black matrix extending along a second direction and covering the insulating spacers is formed on the substrate. A passivation layer is formed for covering the first conductive black matrix, the color film layer, the insulating spacers and the second conductive black matrix. A transparent conductive layer is formed on the passivation layer.