Invention Application
- Patent Title: METHOD FOR INCREASING THE EFFICIENCY OF INDUCING PLURIPOTENT STEM CELLS
- Patent Title (中): 增加诱导大脑干细胞效率的方法
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Application No.: US13808596Application Date: 2012-03-31
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Publication No.: US20130302893A1Publication Date: 2013-11-14
- Inventor: Duanqing Pei , Tao Wang
- Applicant: Duanqing Pei , Tao Wang
- Applicant Address: CN Guangdong
- Assignee: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES
- Current Assignee: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES
- Current Assignee Address: CN Guangdong
- Priority: CN201110323779.7 20111021
- International Application: PCT/CN2012/073375 WO 20120331
- Main IPC: C12N5/0775
- IPC: C12N5/0775

Abstract:
The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.
Public/Granted literature
- US09163217B2 Method for increasing the efficiency of inducing pluripotent stem cells Public/Granted day:2015-10-20
Information query
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