发明申请
- 专利标题: AUTOMATED CTC DETECTION
- 专利标题(中): 自动CTC检测
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申请号: US14118701申请日: 2012-04-13
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公开(公告)号: US20140106388A1公开(公告)日: 2014-04-17
- 发明人: Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
- 申请人: Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
- 申请人地址: DE Munich
- 专利权人: SIEMENS AKTIENGESELLSCHAFT
- 当前专利权人: SIEMENS AKTIENGESELLSCHAFT
- 当前专利权人地址: DE Munich
- 优先权: DE102011076221.3 20110520
- 国际申请: PCT/EP2012/056802 WO 20120413
- 主分类号: G01N33/50
- IPC分类号: G01N33/50
摘要:
An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.
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