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    AUTOMATED CTC DETECTION
    3.
    发明申请
    AUTOMATED CTC DETECTION 审中-公开
    自动CTC检测

    公开(公告)号：US20140106388A1

    公开(公告)日：2014-04-17

    申请号：US14118701

    申请日：2012-04-13

    申请人： Joachim Bangert ,  Katja Friedrich ,  Walter Gumbrecht ,  Karsten Hiltawsky ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Joachim Bangert ,  Katja Friedrich ,  Walter Gumbrecht ,  Karsten Hiltawsky ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5091 ,  C12Q1/6806 ,  G01N1/30

    摘要： An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.

    摘要翻译： 实施方案涉及用于检测与液体混合的体液（例如血液，尿液）或组织样品（例如骨髓）中的活的循环或播散细胞的方法和阵列。 该方法包括：a）通过适于保留待检测细胞的多孔膜过滤液体样品，使待检测细胞停留在膜表面的至少一部分上，样品液体通过 膜; b）施加含有用于用第一标记标记待检测细胞的第一试剂的第一处理液; c）将所述工艺液体在所述膜上孵育预定时间段，其中所述待检测的细胞被标记; 以及d）检测在膜的表面上待检测的标记细胞。

    METHOD AND ARRANGEMENT FOR CALIBRATING A SENSOR ELEMENT
    6.
    发明申请
    METHOD AND ARRANGEMENT FOR CALIBRATING A SENSOR ELEMENT 审中-公开
    用于校准传感器元件的方法和装置

    公开(公告)号：US20100248243A1

    公开(公告)日：2010-09-30

    申请号：US12743316

    申请日：2008-10-29

    申请人： Katja Friedrich ,  Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel ,  Renee Weber

    发明人： Katja Friedrich ,  Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel ,  Renee Weber

    IPC分类号： C12Q1/68 ,  C12M1/40

    CPC分类号： C12Q1/6837 ,  C12Q2527/107

    摘要： A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p] Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)

    摘要翻译： 公开了一种用于校准具有固定化探针寡核苷酸的传感器元件的方法，通过该探针寡​​核苷酸可以通过传感器检测靶核酸（Z）的结合。 在至少一个实施方案中，该方法包括：a）使传感器元件与对照核酸（K）接触，其熔解温度Tm（K）小于靶核酸的解链温度Tm（Z） 酸（Z）; b）在温度T [p] Tm（K）并且在温度T [n]处检测负控制信号。 根据本发明的至少一个实施方案的改进，在测量温度T [mess]测量目标核酸（Z）的测量信号，其中Tm（K）

    Process for concentrating nucleic acid molecules
    8.
    发明授权
    Process for concentrating nucleic acid molecules 有权
    浓缩核酸分子的方法

    公开(公告)号：US08975017B2

    公开(公告)日：2015-03-10

    申请号：US12734934

    申请日：2008-11-03

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： C12Q1/68 ,  G01N33/48

    CPC分类号： C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6837 ,  Y10T436/143333 ,  C12Q2527/107 ,  C12Q2537/125 ,  C12Q2565/543

    摘要： A process concentrates nucleic acid molecules to be detected of a sample on a surface, with capture molecules that specifically bind the nucleic acid molecules. A support material has a capture probe that can specifically be linked to the nucleic acid molecules to be detected to give complexes. The support material and the nucleic acid molecules of the sample are incubated to form the complexes. The complexes are moved to the surface. At least one portion of the complexes becomes bound to the capture molecules via the capture probe.

    摘要翻译： 一种方法将待检测的样品的核酸分子浓缩在表面上，其中捕获分子特异性结合核酸分子。 载体材料具有捕获探针，其可以特异性地连接到待检测的核酸分子以得到复合物。 将样品的载体材料和核酸分子孵育以形成复合物。 络合物移动到表面。 复合物的至少一部分经由捕获探针与捕获分子结合。

    Apparatus and method comprising a sensor array and a porous plunger and use thereof
    9.
    发明授权
    Apparatus and method comprising a sensor array and a porous plunger and use thereof 有权
    包括传感器阵列和多孔柱塞的装置和方法及其用途

    公开(公告)号：US08753582B2

    公开(公告)日：2014-06-17

    申请号：US12867561

    申请日：2009-02-09

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： G01N35/00 ,  G01N31/22 ,  G01N33/52 ,  B01L3/00 ,  G01N31/16 ,  B01L99/00 ,  G01N30/62 ,  G01N30/95 ,  G01N33/00 ,  C12M1/34 ,  C12M3/00

    CPC分类号： G01N33/5438 ,  B01J2219/00382 ,  B01J2219/00527 ,  B01J2219/00704 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/0427 ,  G01N27/27 ,  G01N27/3277

    摘要： Disclosed is an apparatus that is used for detecting liquids or substances from liquids and includes a plunger that has a porous plunger layer which is pressed onto a sensor array. Each sensor of the sensor array is surrounded by elevations which spatially separate the sensors from each other like walls. In at least one embodiment, when the plunger layer is pressed onto the sensor array, the walls are pressed into the pores of the plunger layer. Liquid-tight connections are created between the walls and the plunger layer while liquid remains over the sensors. The liquid can be measured. In at least one embodiment, when there is direct mechanical contact between the plunger layer and the sensors, the liquid over the sensors is located in open pores on the surface of the plunger layer. No liquid flows between the pores and across the walls when pressure is applied to the plunger layer such that closed reaction chambers are created.

    摘要翻译： 公开了一种用于从液体中检测液体或物质的装置，包括具有压在传感器阵列上的多孔柱塞层的柱塞。 传感器阵列的每个传感器被高度包围，空间上将传感器彼此像墙壁分开。 在至少一个实施例中，当柱塞层被压到传感器阵列上时，壁压入柱塞层的孔中。 在壁和柱塞层之间产生液密连接，而液体保留在传感器上方。 可以测量液体。 在至少一个实施例中，当柱塞层和传感器之间存在直接机械接触时，传感器上的液体位于柱塞层表面上的开放孔中。 当压力施加到柱塞层时，没有液体流过孔隙和壁之间，从而形成封闭的反应室。