Invention Application
US20140127777A1 METHOD FOR STABILIZATION OF PROTEINS USING NON-NATURAL AMINO ACIDS
审中-公开
使用非天然氨基酸稳定蛋白质的方法
- Patent Title: METHOD FOR STABILIZATION OF PROTEINS USING NON-NATURAL AMINO ACIDS
- Patent Title (中): 使用非天然氨基酸稳定蛋白质的方法
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Application No.: US14066371Application Date: 2013-10-29
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Publication No.: US20140127777A1Publication Date: 2014-05-08
- Inventor: David A. Tirrell , Yi Tang
- Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
- Applicant Address: US CA PASADENA
- Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
- Current Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
- Current Assignee Address: US CA PASADENA
- Main IPC: C12N9/96
- IPC: C12N9/96 ; C07K2/00
Abstract:
The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
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