发明申请
- 专利标题: SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT
- 专利标题(中): SPHINGOMYELIN测量方法和测量套件
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申请号: US14232427申请日: 2012-07-25
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公开(公告)号: US20140162300A1公开(公告)日: 2014-06-12
- 发明人: Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
- 申请人: Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
- 申请人地址: JP Chuo-ku, Tokyo
- 专利权人: KYOWA MEDEX CO., LTD.
- 当前专利权人: KYOWA MEDEX CO., LTD.
- 当前专利权人地址: JP Chuo-ku, Tokyo
- 优先权: JP2011-166458 20110729
- 国际申请: PCT/JP2012/068807 WO 20120725
- 主分类号: C12Q1/61
- IPC分类号: C12Q1/61
摘要:
Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.