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公开(公告)号：US20220403442A1
公开(公告)日：2022-12-22
申请号：US17770146
申请日：2020-10-20
发明人： Simon Sam Matoori , David J. Mooney
摘要： Described herein are compositions that are suitable for use in analyte sensing in biological samples and in medical diagnostics. The compositions include an oxidase capable of oxidizing the analyte of interest to produce hydrogen peroxide, a peroxidase, and a chemical compound, such as a near-infrared fluorescent compound, that is a substrate for the peroxidase. The oxidase, the peroxidase, and the chemical compound are encapsulated by vesicle that includes a lipid or polymeric bilayer, such as liposome and polymersome. The peroxidase catalyzes the oxidation of the chemical compound by hydrogen peroxide. Methods of analyte sensing in biological samples using these compositions, and methods of preparing the compositions are also described.
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公开(公告)号：US20210332410A1
公开(公告)日：2021-10-28
申请号：US17363738
申请日：2021-06-30
申请人： AXIS-SHIELD AS
摘要： The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided.
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公开(公告)号：US20200063185A1
公开(公告)日：2020-02-27
申请号：US16530322
申请日：2019-08-02
发明人： Douglas Matje , Ian Gibbons , Paul Patel , Elizabeth A. Holmes
摘要： Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.
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公开(公告)号：US10175245B2
公开(公告)日：2019-01-08
申请号：US14543026
申请日：2014-11-17
摘要： The present invention provides methods for continuous measurement of triglyceride digestibility during a meal. A stable isotope labeled triglyceride and a free fatty acid tracer are added to the meal. The ratio between a ratio of isotope labeled fatty acid produced after digestion to free fatty acid tracer represents the percentage of digestion of triglycerides by lipase from the pancreas.
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公开(公告)号：US10077286B2
公开(公告)日：2018-09-18
申请号：US14395449
申请日：2013-04-19
发明人： Motoko Ohta
CPC分类号： C07K1/14 , C12Q1/26 , C12Q1/44 , C12Q1/485 , C12Q1/61 , G01N33/6893 , G01N33/92 , G01N2800/32 , G01N2800/7085
摘要： Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
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公开(公告)号：US20180245129A1
公开(公告)日：2018-08-30
申请号：US15967216
申请日：2018-04-30
IPC分类号： C12Q1/61 , G01N33/574 , G01N33/542 , G01N33/50 , A61K49/00 , A61K41/00
CPC分类号： C12Q1/61 , A61K41/0071 , A61K49/0017 , A61K49/0032 , A61K49/0036 , G01N33/5011 , G01N33/542 , G01N33/57484
摘要： The present invention is directed to a phospholipid-based NIR molecular beacon, having a phospholipid moiety; with an NIR fluorophore moiety covalently linked to a phospholipid glycerol backbone and a quencher moiety covalently linked to the phospholipid glycerol backbone. Additionally, provided herein is methods of analyzing a sample for the presence of a phospholipase and methods of identifying the activity of a phospholipase in vivo utilizing phospholipid-based NIR molecular beacon.
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公开(公告)号：US20170306389A1
公开(公告)日：2017-10-26
申请号：US15510059
申请日：2015-09-18
发明人： Shigeru UEDA , Shinichi SAKASEGAWA
摘要： The present invention provides a measuring method for at least one of a kinase forward reaction substrate, a phosphorylated product thereof, and a precursor thereof, and includes a step of conducting an enzymatic cycling reaction by bringing at least a kinase, a first nucleotide coenzyme of the kinase, and a second nucleotide coenzyme having a different nucleoside moiety from the first nucleotide coenzyme into contact with a sample; a step of detecting a signal corresponding to a change of at least one of the first nucleotide coenzyme and a conversion product thereof, and the second nucleotide coenzyme and a conversion product thereof; and (3) a step of calculating, on the basis of the detected change of the signal, an amount of the kinase forward reaction substrate and/or the phosphorylated product thereof contained in the sample.
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8.发明授权Isovalerylglycine as biomarker for the predispositon for weight gain and obesity 有权异戊酰甘氨酸作为体重增加和肥胖的前体的生物标志物公开(公告)号：US09500660B2
公开(公告)日：2016-11-22
申请号：US14649072
申请日：2013-11-25
申请人： Nestec S.A.
发明人： Francois-Pierre Martin , Claire L. Boulange , Ivan Montoliu Rora , Sebastiano Collino , Marc-Emmanuel Dumas , Elaine Holmes , Serge Andre Dominique Rezzi , Jeremy Nicholson , Sunil Kochhar
CPC分类号： G01N33/6893 , C12Q1/61 , C12Q2565/633 , G01N24/08 , G01N33/50 , G01N33/6806 , G01N2800/044 , G01N2800/50 , G01N2800/60 , G01N2800/70
摘要： The present invention relates generally to the field of nutrition and health. In particular, the present invention relates to a new biomarker, its use and a method that allows it to diagnose the likelihood to resist diet induced weight gain, and/or to be susceptible to a diet induced weight gain. For example, the biomarker may be isovalerylglycine.
摘要翻译： 本发明一般涉及营养和健康领域。 特别地，本发明涉及新的生物标志物，其用途和方法，其允许其诊断抵抗饮食诱导的体重增加的可能性，和/或易于饮食诱导的体重增加。 例如，生物标志物可以是异戊酰甘氨酸。
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9.发明授权公开(公告)号：US09051600B2
公开(公告)日：2015-06-09
申请号：US14232427
申请日：2012-07-25
CPC分类号： C12Q1/61 , C12Q1/28 , C12Q1/44 , C12Y101/03017 , C12Y301/01005 , C12Y301/04004 , G01N33/92 , G01N2333/92 , G01N2405/08
摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
摘要翻译： 本发明提供了简单且准确地测量样品中鞘磷脂的方法，因此提供了一种试剂盒。 该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢 使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。
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公开(公告)号：US20140162300A1
公开(公告)日：2014-06-12
申请号：US14232427
申请日：2012-07-25
IPC分类号： C12Q1/61
CPC分类号： C12Q1/61 , C12Q1/28 , C12Q1/44 , C12Y101/03017 , C12Y301/01005 , C12Y301/04004 , G01N33/92 , G01N2333/92 , G01N2405/08
摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
摘要翻译： 本发明提供了简单且准确地测定样品中鞘磷脂的方法及其用途。 该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢 使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。
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