Provided are an optical pickup and an optical information storage system including the same. The optical pickup includes a diffracting element that splits incident light into main light and a plurality of sub light. The diffracting element has at least three regions including first through third regions. A second region is disposed between a first region and a third region and has a phase difference from the first and third regions. The optical pickup detects a tracking error signal by using light diffracted by the diffracting element.