发明申请
- 专利标题: PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM
- 专利标题(中): 生产重组蛋白作为可溶形式的方法
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申请号: US14476624申请日: 2014-09-03
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公开(公告)号: US20150087047A1公开(公告)日: 2015-03-26
- 发明人: Satoshi INOUYE , Yuiko SAHARA
- 申请人: JNC CORPORATION
- 申请人地址: JP Tokyo
- 专利权人: JNC CORPORATION
- 当前专利权人: JNC CORPORATION
- 当前专利权人地址: JP Tokyo
- 优先权: JP2006253045 20060919
- 主分类号: C07K14/31
- IPC分类号: C07K14/31 ; C12N9/02
摘要:
A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.
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