发明申请
- 专利标题: Method for Detecting Target Nucleic Acid
- 专利标题(中): 检测靶核酸的方法
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申请号: US14477550申请日: 2014-09-04
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公开(公告)号: US20150111774A1公开(公告)日: 2015-04-23
- 发明人: Kousuke Niwa , Toshikazu Hirota
- 申请人: NGK INSULATORS, LTD.
- 申请人地址: JP Aichi-ken
- 专利权人: NGK INSULATORS, LTD.
- 当前专利权人: NGK INSULATORS, LTD.
- 当前专利权人地址: JP Aichi-ken
- 优先权: JP2012-231071 20121018
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
The disclosure of this Description includes: a step of preparing a solid-phase body provided with a detection probe having a predetermined nucleotide sequence; a step of preparing a signaling target nucleic acid having a signal generating part for detecting the target nucleic acid; a step of bringing the signaling target nucleic acid, the detection probe, and an oligonucleotide being a mediator having a first site that hybridizes specifically with the target nucleic acid and a second site (preferably 20 to 50 nucleotides or more preferably 20 to 25 nucleotides in length) that hybridizes specifically with a part having the predetermined nucleotide sequence of the detection probe into contact with one another so that a hybridization product of these components can be formed; and a step of obtaining data based on the signal generating part of the signaling target nucleic acid on the solid-phase body.
公开/授权文献
- US20170306393A9 Method for Detecting Target Nucleic Acid 公开/授权日:2017-10-26
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