A diagnostic system for an engine includes: a plurality of cylinders, in-cylinder injectors, port injectors and an electronic control unit. The electronic control unit configured to make an abnormality diagnosis of an air-fuel ratio due to the in-cylinder injectors and then to make an abnormality diagnosis of the air-fuel ratio due to the port injectors. The electronic control unit is configured to make an abnormality diagnosis of the air-fuel ratio due to the in-cylinder injectors in an operating situation in which fuel is injected from only the in-cylinder injectors, and to make an abnormality diagnosis of the air-fuel ratio due to the port injectors by increasing a ratio of an injection amount of the port injectors when the electronic control unit has diagnosed that there is an abnormality of the air-fuel ratio in an operating situation in which fuel is injected from both the in-cylinder injectors and the port injectors.