发明申请
- 专利标题： AGENT AND KIT FOR ISOLATING NUCLEIC ACIDS
- 专利标题（中）： 用于分离核酸的试剂和试剂盒
-
申请号： US14645174申请日： 2015-03-11
-
公开(公告)号： US20150184149A1公开(公告)日： 2015-07-02
- 发明人: Pei-Shin JIANG , Kun-Chan WU , Yu-Ting SU , Chia-Yun LIN , Siou-Cing SU , Yuh-Jiuan LIN
- 申请人： Pei-Shin JIANG , Kun-Chan WU , Yu-Ting SU , Chia-Yun LIN , Siou-Cing SU , Yuh-Jiuan LIN
- 申请人地址： TW Chutung
- 专利权人： INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
- 当前专利权人： INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
- 当前专利权人地址： TW Chutung
- 优先权： TW98135732 20091022
- 主分类号： C12N15/10
- IPC分类号： C12N15/10
摘要：
A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.