发明申请
US20150203548A1 Single-Domain Antibodies with Improved Thermal Stability Under Cytoplasmic Expression
审中-公开
具有改善细胞质表达下热稳定性的单域抗体
- 专利标题： Single-Domain Antibodies with Improved Thermal Stability Under Cytoplasmic Expression
- 专利标题（中）： 具有改善细胞质表达下热稳定性的单域抗体
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申请号： US14158208申请日： 2014-01-17
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公开(公告)号： US20150203548A1公开(公告)日： 2015-07-23
- 发明人: Daniel Zabetakis , George P. Anderson , Ellen R. Goldman , Kendrick Turner , P. Audrey Brozozog Lee
- 申请人： Daniel Zabetakis , George P. Anderson , Ellen R. Goldman , Kendrick Turner , P. Audrey Brozozog Lee
- 申请人地址： US VA Arlington
- 专利权人： The Government of the United States of America, as represented by the Secretary of the Navy
- 当前专利权人： The Government of the United States of America, as represented by the Secretary of the Navy
- 当前专利权人地址： US VA Arlington
- 主分类号： C07K14/47
- IPC分类号： C07K14/47 ; C07K16/12
摘要：
Methods of producing a single-domain antibody (sdAb) include causing a bacteria to express the sdAb into cytoplasm of the bacteria, wherein the sdAb is expressed as a fusion protein with the acid tail of α-synuclein; and then purifying the sdAb, wherein the fusion protein is expressed free of a periplasmic location tag. Such antibodies have the unexpected ability to refold after thermal denaturation.
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