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1.发明申请Single-Domain Antibodies with Improved Thermal Stability Under Cytoplasmic Expression 审中-公开具有改善细胞质表达下热稳定性的单域抗体公开(公告)号：US20150203548A1
公开(公告)日：2015-07-23
申请号：US14158208
申请日：2014-01-17
申请人： Daniel Zabetakis , George P. Anderson , Ellen R. Goldman , Kendrick Turner , P. Audrey Brozozog Lee
发明人： Daniel Zabetakis , George P. Anderson , Ellen R. Goldman , Kendrick Turner , P. Audrey Brozozog Lee
CPC分类号： C07K14/47 , C07K16/1271 , C07K16/1278 , C07K16/16 , C07K2317/569 , C07K2317/94 , C07K2319/00
摘要： Methods of producing a single-domain antibody (sdAb) include causing a bacteria to express the sdAb into cytoplasm of the bacteria, wherein the sdAb is expressed as a fusion protein with the acid tail of α-synuclein; and then purifying the sdAb, wherein the fusion protein is expressed free of a periplasmic location tag. Such antibodies have the unexpected ability to refold after thermal denaturation.
摘要翻译： 产生单结构域抗体（sdAb）的方法包括使细菌将细胞表达为细菌的细胞质，其中sdAb与α-突触核蛋白的酸性尾端一起表达为融合蛋白; 然后纯化sdAb，其中融合蛋白被表达为没有周质位置标签。 这种抗体在热变性后具有突然的重折叠能力。
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公开(公告)号：US08658372B2
公开(公告)日：2014-02-25
申请号：US11307399
申请日：2006-02-06
IPC分类号： G01N33/53
CPC分类号： G01N33/56916 , G01N33/5308 , G01N33/54366 , G01N33/554 , G01N33/56911 , G01N33/56983
摘要： A method of biochemical identification by: providing a plurality of capture species bound to one or more substrates and suspected of having one or more biological targets affinity bound to at least one capture species; detecting which capture species contain bound biological targets to generate a binding pattern; and identifying the biological target based on the binding pattern. The capture species are independently selected from the group consisting of antimicrobial peptides, cytotoxic peptides, antibiotics, and combinations thereof. A device having the capture species bound to the substrates. At least two of the capture species are capable of multi-specific binding to one or more biological targets and may have overlapping but not identical affinity properties.
摘要翻译： 生物化学鉴定的方法是：提供与一种或多种底物结合的多种捕获物质，并怀疑具有与至少一种捕获物质结合的一种或多种生物靶标; 检测哪些捕获物种含有结合的生物靶标以产生结合模式; 以及基于所述结合模式识别所述生物学目标。 捕获物种独立地选自抗微生物肽，细胞毒性肽，抗生素及其组合。 具有捕获物质与基底结合的装置。 至少两种捕获物质能够与一种或多种生物靶标进行多特异性结合，并且可以具有重叠但不相同的亲和性质。
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3.发明授权Method of controlling quantum dot photoluminescence and other intrinsic properties through biological specificity 失效通过生物学特异性控制量子点光致发光和其他固有性质的方法公开(公告)号：US07858386B2
公开(公告)日：2010-12-28
申请号：US11683226
申请日：2007-03-07
申请人： Igor L Medintz , Hedi M Mattoussi , Moungi G Bawendi , J Matthew Mauro , George P Anderson , Thomas Pons
发明人： Igor L Medintz , Hedi M Mattoussi , Moungi G Bawendi , J Matthew Mauro , George P Anderson , Thomas Pons
IPC分类号： G01N21/64 , G01N33/551 , C12Q1/37
CPC分类号： G01N33/588 , B82Y15/00 , C12Q1/6825 , G01N33/542 , Y10T436/143333
摘要： A method of controllably changing an intrinsic property of a quantum dot by using a biological entity, either attached or in close proximity to the quantum dot, and changing the state of biological entity with a controllable mechanism. The change in state of the biological entity controllably changes the intrinsic property of the quantum dot. The photoluminescence emission of quantum dots can be controlled by the present method. The methods disclosed include controlling the magnitude of QD photoluminescence as well as turning the photoluminescence on/off. The methods disclosed include using the same biological control architecture to control other intrinsic QD properties such as charge state, magnetic or other property.
摘要翻译： 通过使用附着或非常接近量子点的生物实体可控地改变量子点的固有特性的方法，并以可控机制改变生物实体的状态。 生物体的状态变化可以控制地改变量子点的固有特性。 量子点的光致发光可通过本方法进行控制。 所公开的方法包括控制QD光致发光的大小以及打开/关闭光致发光。 所公开的方法包括使用相同的生物学控制结构来控制其它固有QD特性，例如电荷状态，磁性或其他性质。
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公开(公告)号：US20100308275A1
公开(公告)日：2010-12-09
申请号：US12318028
申请日：2008-12-19
IPC分类号： C09K11/02
CPC分类号： B82Y15/00 , G01N33/551 , G01N33/588
摘要： The ionic conjugates include an inorganic particle electrostatically associated with a macromolecule which can interact specifically with predetermined chemical species or biological targets.
摘要翻译： 离子共轭物包括与大分子静电相关的无机颗粒，其可以与预定的化学物质或生物靶相互作用。
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公开(公告)号：US07470379B2
公开(公告)日：2008-12-30
申请号：US11080946
申请日：2005-03-16
CPC分类号： B82Y15/00 , G01N33/551 , G01N33/588
摘要： The ionic conjugates include an inorganic particle electrostatically associated with a macromolecule which can interact specifically with predetermined chemical species or biological targets.
摘要翻译： 离子共轭物包括与大分子静电相关的无机颗粒，其可以与预定的化学物质或生物靶相互作用。
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公开(公告)号：US09778265B2
公开(公告)日：2017-10-03
申请号：US14688010
申请日：2015-04-16
IPC分类号： G01N33/68 , C07K14/00 , C12N15/62 , G01N33/531 , G01N33/543 , C07K19/00 , C07K16/16
CPC分类号： G01N33/68 , C07K14/00 , C07K16/16 , C07K19/00 , C07K2317/569 , C07K2319/73 , C12N15/62 , G01N33/531 , G01N33/54393 , G01N2333/42
摘要： Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.
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7.发明申请Charged Peptide Appendage to Facilitate Oriented Protein Covalent Immobilization 有权带电肽附件促进定向蛋白质共价固定公开(公告)号：US20160305948A1
公开(公告)日：2016-10-20
申请号：US14688010
申请日：2015-04-16
IPC分类号： G01N33/68
CPC分类号： G01N33/68 , C07K14/00 , C07K16/16 , C07K19/00 , C07K2317/569 , C07K2319/73 , C12N15/62 , G01N33/531 , G01N33/54393 , G01N2333/42
摘要： Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.
摘要翻译： 具有带正电荷结构域的蛋白质的遗传融合，例如单结构域抗体（sdAb）增强了活性蛋白在期望取向上的固定化。
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8.发明授权公开(公告)号：US09267930B2
公开(公告)日：2016-02-23
申请号：US13653474
申请日：2012-10-17
CPC分类号： G01N33/227 , C07K16/44 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/569 , C07K2317/622 , C07K2317/92 , C07K2317/94 , G01N33/5308
摘要： Described herein are new recognition elements (antibodies or functional fragments thereof) that effectively bind to trinitrotoluene (TNT). Also disclosed is a single chain fragment recognition element.
摘要翻译： 本文描述了有效结合三硝基甲苯（TNT）的新的识别元件（抗体或其功能片段）。 还公开了单链片段识别元件。
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公开(公告)号：US09201010B2
公开(公告)日：2015-12-01
申请号：US12431784
申请日：2009-04-29
CPC分类号： G01N21/6428 , B82Y20/00 , B82Y30/00 , C07D471/06 , C09K11/06 , C09K2211/1416 , C09K2211/1433 , C09K2211/1466 , G01N2021/6439 , G01N2021/6441 , H05B33/14
摘要： A nanoparticle having a surfactant shell with a hydrophilic outer surface and a hydrophobic inner surface and an organic chromophore and a polymer having aromatic groups within the surfactant shell. A method of making nanoparticles by: emulsifying an aqueous composition of a surfactant and an organic solution of a monomer and an organic chromophore to form micelles of the monomer and the chromophore inside a surfactant shell; and polymerizing the monomer. A method of: reacting a ω-bromoalkyl acid with acryloyl acid lithium salt, and reacting the product with sodium hydride to produce an acryloyloxyalkyl carboxylic acid sodium salt. The compound shown below.
摘要翻译： 具有亲水外表面和疏水性内表面的表面活性剂外壳和有机发色团的纳米颗粒和在表面活性剂外壳内具有芳族基团的聚合物。 一种通过以下方法制备纳米颗粒：将表面活性剂的水性组合物和单体和有机发色团的有机溶液乳化，以在表面活性剂壳内形成单体和发色团的胶束; 并使单体聚合。 一种使ω-溴代烷基酸与丙烯酰基锂盐反应并使产物与氢化钠反应产生丙烯酰氧基烷基羧酸钠盐的方法。 化合物如下所示。
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公开(公告)号：US20110319601A1
公开(公告)日：2011-12-29
申请号：US13229177
申请日：2011-09-09
CPC分类号： B82Y15/00 , G01N33/551 , G01N33/588
摘要： The ionic conjugates include an inorganic particle electrostatically associated with a macromolecule which can interact specifically with predetermined chemical species or biological targets.
摘要翻译： 离子共轭物包括与大分子静电相关的无机颗粒，其可以与预定的化学物质或生物靶相互作用。
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