The process includes pretreating the biomass to provide a fluidized biomass. The fluidized biomass is then subjected to high frequency pulses and shear forces without denaturing/degrading the individual components of the biomass. The biomass is then subjected to compressive force to separate a first liquid fraction from a first fractionated biomass. The first fractionated biomass may again then be subjected to the same high frequency pulses and shear forces as previously, particularly if there are hemicellulose and/or sugars still present in the first fractionated biomass. Compressive forces are used to separate a second liquid fraction from a second fractionated biomass. The second fractionated biomass is subjected to oxidation such as with hydrogen peroxide and base at a pH above 7. The second fractioned biomass is then subjected to compressive forces to provide water soluble lignin which may be precipitated by reducing the pH.