A calibration source for a gamma-ray spectrometer is provided. The calibration source comprises a scintillator body having a cavity in which a radioactive material is received. The scintillator body may be generally cuboid and the cavity may be formed by a hole drilled into the scintillator body. The radioactive material comprises a radioactive isotope having a decay transition associated with emission of a radiation particle and a gamma-ray having a known energy e.g. Na-22. A photodetector, for example a silicon photomultiplier, is optically coupled to the scintillator body and arranged to detect scintillation photons generated when radiation particles emitted from the radioactive material interact with the surrounding scintillator bod. A gating circuit is arranged to receive detection signals from the photodetector and to generate corresponding gating signals for a data acquisition circuit of an associated gamma-ray spectrometer to indicate that gamma-ray detections in the gamma-ray spectrometer occurring within a time window defined by the gating signal are associated with a decay transition in the radioactive isotope. Thus a calibration source is provided based around a simple scintillator body design. Furthermore, the radioactive material may be introduced into the scintillator body in a separate step after manufacture of the scintillator body, thereby reducing the risk of radioactive contamination during manufacture.