The present disclosure relates to the field of liquid crystal display, and particularly to a frame seal structure on a substrate, a substrate, and a liquid crystal display device. The frame seal structure comprises a primary seal and an assembly process dummy seal. The assembly process dummy seal is formed in an area between the primary seal and an edge of the substrate, and consists of a plurality of sub-seals which are arranged discontinuously. The present frame seal structure is a novel frame seal structure which is proposed with respect to drawbacks of the process dummy seal during assembling with a VIF process in a liquid crystal display device with post spacers. By virtue of the novel frame seal, the peripheral region of the substrate will not be pressed airtight during assembling, so that the gas can be discharged, and poor sealing in a certain area is avoided. Thus the present disclosure can solve the problem of poor pressing of frame seal, particularly the problems like seal brush and seal narrow, and can be better adapted to the VIF process and improve the yield. (FIG. 1)