Invention Application
US20160138047A1 IMPROVED POLYNUCLEOTIDE SEQUENCES ENCODING TALE REPEATS
审中-公开
改进多核苷酸序列编码TALE REPEATS
- Patent Title: IMPROVED POLYNUCLEOTIDE SEQUENCES ENCODING TALE REPEATS
- Patent Title (中): 改进多核苷酸序列编码TALE REPEATS
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Application No.: US14898148Application Date: 2014-06-13
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Publication No.: US20160138047A1Publication Date: 2016-05-19
- Inventor: Philippe DUCHATEAU , Sylvain ARNOULD , Julianne SMITH
- Applicant: CELLECTIS
- Priority: DKPA201370321 20130614
- International Application: PCT/EP2014/062405 WO 20140613
- Main IPC: C12N15/90
- IPC: C12N15/90 ; C12N15/86 ; C12N7/00 ; C12N9/22 ; C07K14/00

Abstract:
The present invention is in the field of the gene editing molecular tools. The present invention relates to rewritten nucleic acid sequences encoding repeated DNA recognition motifs of TALE (Transcription Activator-Like Effector) proteins. These nucleic acid sequences allow assembly and cloning of TALE repeats in any type of vectors, especially viral vectors. The invention thereby contributes to improving gene targeting in cells using TALE derived proteins, in particular for genetic regulation or modification. The present invention is particularly drawn to virus mediated transformation methods, by providing vectors, compositions and kits including said new nucleic acid sequences.
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