A display panel includes: an array substrate, which includes a gate line and a data line crossing to each other, a thin film transistor disposed in a crossing region of the gate line and the data line; a color filter substrate, cell-assembled with the array substrate; and a spacer, located between the array substrate and the color filter substrate, wherein a contact face between the spacer and the array substrate is positioned in a region of the gate line and/or a region of the data line except a region where the thin film transistor is located. A manufacturing method of a display panel and a display device also are provided.