Invention Application
- Patent Title: METHODS AND SYSTEMS FOR PURE DYE INSTRUMENT NOMALIZATION
- Patent Title (中): 纯粹的仪器仪表化方法和系统
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Application No.: US15016713Application Date: 2016-02-05
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Publication No.: US20160237474A1Publication Date: 2016-08-18
- Inventor: Jeffrey Marks
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N21/64

Abstract:
The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.
Public/Granted literature
- US09809849B2 Methods and systems for pure dye instrument normalization Public/Granted day:2017-11-07
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