发明申请
- 专利标题： NON-RIBOSOMAL PROTEIN SYNTHESIS PIGMENT FUSION PEPTIDES
- 专利标题（中）： 非蛋白质蛋白合成染料融合蛋白
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申请号： US15026734申请日： 2014-10-02
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公开(公告)号： US20160238611A1公开(公告)日： 2016-08-18
- 发明人: Roland EILS , Barbara DI VENTURA , Lorenz ADLUNG , Katharina GENREITH , Tim HEINEMANN , Hannah MEYER , Dominik NIOPEK , Fanny GEORGI , Ralf BEER , Tania Christina CHRISTIANSEN , Konrad HERBST , Nikolaos IGNATIADIS , Ilia KATS , Nils KURZAWA , Johanna MEICHSNER , Sophie RABE , Anja RIEDEL , Joshua SACHS , Julia Patricia SCHESSNER , Florian SCHMIDT , Philipp Darius Konstantin WALCH
- 申请人： Deutsches Krebsforschungszentrum , Ruprecht-Karls-Universität Heidelberg
- 优先权： EP13187133.7 20131002
- 国际申请： PCT/EP2014/071155 WO 20141002
- 主分类号： G01N33/58
- IPC分类号： G01N33/58 ; C12Q1/02 ; C12N9/16 ; C12P21/00 ; C07K2/00 ; C12N9/00
摘要：
The present invention relates to a polypeptide or polypeptide complex, comprising at least one non-ribosomal peptide synthesis (NRPS) amino acid module functionally connected to at least one pigment module. The present invention further relates to a labeled oligopeptide comprising a non-naturally attached NRPS pigment or/and polyketide pigment, to a polynucleotide encoding a fusion polypeptide, a vector, preferably an expression vector, comprising the polynucleotide of the present invention and to a host cell comprising the polypeptide or polypeptide complex and/or the polynucleotide, and/or the vector according to the present invention. Moreover, the present invention relates to in vitro and in vivo method of producing a labeled oligopeptide, as well as to methods of optimizing the same.