公开(公告)号：US20160238611A1

公开(公告)日：2016-08-18

申请号：US15026734

申请日：2014-10-02

申请人： Deutsches Krebsforschungszentrum ,  Ruprecht-Karls-Universität Heidelberg

发明人： Roland EILS ,  Barbara DI VENTURA ,  Lorenz ADLUNG ,  Katharina GENREITH ,  Tim HEINEMANN ,  Hannah MEYER ,  Dominik NIOPEK ,  Fanny GEORGI ,  Ralf BEER ,  Tania Christina CHRISTIANSEN ,  Konrad HERBST ,  Nikolaos IGNATIADIS ,  Ilia KATS ,  Nils KURZAWA ,  Johanna MEICHSNER ,  Sophie RABE ,  Anja RIEDEL ,  Joshua SACHS ,  Julia Patricia SCHESSNER ,  Florian SCHMIDT ,  Philipp Darius Konstantin WALCH

IPC分类号： G01N33/58 ,  C12Q1/02 ,  C12N9/16 ,  C12P21/00 ,  C07K2/00 ,  C12N9/00

CPC分类号： G01N33/583 ,  C07K2/00 ,  C12N9/16 ,  C12N9/93 ,  C12P21/00 ,  C12Q1/02 ,  C12Y301/00 ,  C12Y603/02 ,  G01N2440/00 ,  G01N2458/00

摘要： The present invention relates to a polypeptide or polypeptide complex, comprising at least one non-ribosomal peptide synthesis (NRPS) amino acid module functionally connected to at least one pigment module. The present invention further relates to a labeled oligopeptide comprising a non-naturally attached NRPS pigment or/and polyketide pigment, to a polynucleotide encoding a fusion polypeptide, a vector, preferably an expression vector, comprising the polynucleotide of the present invention and to a host cell comprising the polypeptide or polypeptide complex and/or the polynucleotide, and/or the vector according to the present invention. Moreover, the present invention relates to in vitro and in vivo method of producing a labeled oligopeptide, as well as to methods of optimizing the same.

摘要翻译： 本发明涉及多肽或多肽复合物，其包含与至少一种颜料模块功能连接的至少一种非核糖体肽合成（NRPS）氨基酸模块。 本发明还涉及包含非天然连接的NRPS颜料或/和聚酮化合物颜料的标记的寡肽与编码融合多肽的多核苷酸，载体，优选包含本发明的多核苷酸的载体和宿主 包含多肽或多肽复合物和/或多核苷酸的细胞，和/或根据本发明的载体。 此外，本发明涉及产生标记寡肽的体外和体内方法，以及优化其的方法。