发明申请
US20160304932A1 METHOD AND A KIT FOR THE DIAGNOSIS OF HEPATIC DISEASE UTILIZING THE SULFYDRYL-OXIDIZING PROPERTY OF SERUM
审中-公开
用于诊断使用血清的硫代氧化性质的肝病的方法和试剂盒
- 专利标题： METHOD AND A KIT FOR THE DIAGNOSIS OF HEPATIC DISEASE UTILIZING THE SULFYDRYL-OXIDIZING PROPERTY OF SERUM
- 专利标题（中）： 用于诊断使用血清的硫代氧化性质的肝病的方法和试剂盒
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申请号： US15087842申请日： 2016-03-31
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公开(公告)号： US20160304932A1公开(公告)日： 2016-10-20
- 发明人: Jinsong Zhang , Kang Sun
- 申请人： Anhui Agricultural University
- 优先权： CN201510181394.X 20150416
- 主分类号： C12Q1/26
- IPC分类号： C12Q1/26 ; C12Q1/28
摘要：
The present invention refers to a method and a kit for the diagnosis of a hepatic disease utilizing the sulfydryl-oxidizing property of serum. Sulfydryl can be oxidized by serum, due to the QSOXs and other macromolecules or small molecules in serum. The specific activity of QSOXs in serum can be detected by fluorescent quantification using Amplex UltraRed (AUR), horseradish perioxidase, dithiothreitol, and tween as key reagents. The total dithiol oxidated capacity of the serum can be detected by colorimetric quantification using dithiothreitol, dithio-bis-nitrobenzoic acid, and guanidine hydrochloride as key reagents. The present invention shows that the specific activity of QSOXs in serum and the total dithiol oxidated capacity of the serum can be used for the diagnosis of hepatic disease, wherein the total dithiol oxidated capacity of the serum shows better diagnostic effect. The present invention also refers to a kit for the diagnosis of a hepatic disease, comprising a key reagent including phosphate buffer, Amplex UltraRed (AUR), horseradish perioxidase, dithiothreitol, and tween; or including guanidine hydrochloride, dithio-bis-nitrobenzoic acid, and dithiothreitol, etc.
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