发明申请
US20160319248A1 FRUCTOSE AMINO ACID OXIDASE, PREPARATION METHOD AND ENZYME-CONTAINING KIT FOR DETECTING GLYCATED ALBUMIN
有权
纤维素氨基酸氧化酶，用于检测糖化铝的制备方法和含酶包装试剂盒
- 专利标题： FRUCTOSE AMINO ACID OXIDASE, PREPARATION METHOD AND ENZYME-CONTAINING KIT FOR DETECTING GLYCATED ALBUMIN
- 专利标题（中）： 纤维素氨基酸氧化酶，用于检测糖化铝的制备方法和含酶包装试剂盒
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申请号： US15108320申请日： 2014-12-11
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公开(公告)号： US20160319248A1公开(公告)日： 2016-11-03
- 发明人: Bingde ZOU , Jihua ZOU , Yi WANG , Jianghua JIA
- 申请人： NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.
- 申请人地址： CN Ningbo
- 专利权人： NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.
- 当前专利权人： NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.
- 当前专利权人地址： CN Ningbo
- 优先权： CN201310744635.8 20131227
- 国际申请： PCT/CN2014/093580 WO 20141211
- 主分类号： C12N9/06
- IPC分类号： C12N9/06 ; G01N33/68
摘要：
Fructosyl amino acid oxidase is provided, which has an amino acid sequence as shown in SEQID. No. 1 or fructosyl amino acid oxidase having a homology of more than 80% with this amino acid sequence, on a corresponding site of an amino acid selected from following (a) to (f), having one or more amino acid residues conducting a substitution, obtained fructosyl amino acid oxidase having a higher thermostability: (a) 59-site glutamic acid, (b) 98-site glutamic acid, (c) 225-site glycine, (d) 277-site lysine, (e) 285-site glutamic acid, and (f) 355-site aspartic acid. The method for preparing the above oxidase and the test kit containing the enzyme for determining glycated albumin are also provided.