A thin film transistor and manufacturing method thereof, an array substrate, a display panel and a display device are provided. The method includes a step of forming an organic semiconductor layer on a hydrophilic structural layer. The step includes forming a lipophilic material layer on an upper surface of the hydrophilic structural layer; patterning the lipophilic material layer to remove the lipophilic material located at regions other than a region for forming the organic semiconductor layer thereon and remain the lipophilic material located at the region for forming the organic semiconductor layer thereon to form a lipophilic layer; and forming the organic semiconductor layer on the lipophilic layer through a wet process by using the lipophilic organic material.