In various implementations, techniques are described herein to resolve issues related to the location of an EOB NAL unit in an ISO base media file. In various implementations, these techniques include relaxing the requirement that a file include only one EOB NAL unit. These techniques further include providing the EOB NAL unit from the highest layer when reconstructing the bitstream. This EOB NAL unit can be added to a last access unit in the reconstructed bitstream. When the EOB NAL unit from the highest layer is not present, an EOB NAL unit from a lower layer can be provided instead.