The present invention relates to a driving circuit of organic light emitting diode comprising an electricity storage unit having a positive polarity at a first terminal and a negative polarity at a second terminal, and a signal input unit having opposite polarities at the first and second terminals, the signal polarity of the signal input unit being changed according to a preset frequency; a control unit, which causes the first terminal of the signal input unit to transmit negative charges to the anode of the organic light emitting diode and causes the second terminal of the signal input unit to transmit positive charges to the cathode of the organic light emitting diode, when the signal polarity at the first terminal of the signal input unit is negative and the signal polarity of the second terminal thereof is positive, and causes the first terminal of the electricity storage unit to transmit positive charges to the anode of the organic light emitting diode and causes the second terminal of the electricity storage unit to transmit negative charges to the cathode of the organic light emitting diode, when the signal polarity at the first terminal of the signal input unit is positive and the signal polarity of the second terminal thereof is negative. The embodiments of the application can make the cathode of the organic light emitting diode accumulate electrons during the non-excitation period, so that when the electricity storage unit drives the organic light emitting diode to emit light, more electrons can pass through the light emitting layer, so that the excitation subjected by the light emitting layer is improved and more light is emitted.